Biotechnology Archive

Proteomics

Today we have the genome sequences for humans as well as many other animals, plants, fungi, and bacteria. All these data have given scientists a global view of the various genes in humans and others. However, genes are only the …

Immune Memory and Vaccination

Individuals who survive an infection normally become immune to that particular disease, although not to other diseases. This is because the immune system “remembers” foreign antigens, a process called immune memory . Next time the same antigen appears, it triggers …

Monoclonal Antibodies For Clinical Use

    There are many clinical uses for antibodies. They are used in diagnostic procedures (including the ELISA), for pregnancy testing, and to detect the presence of proteins characteristic of particular disease-causing agents. In the future, they may be used …

Antibody Structure

Each antibody consists of four protein subunits, two light chains and two heavy chains , arranged in a Y-shape. Disulfide bonds between cysteine amino acid residues hold the chains together. Each of the light and heavy chains consists of a …

The Great Diversity of Antibodies

Antibodies are proteins that recognize and bind to alien molecules. Because there are an almost infinite variety of possible alien molecules, a correspondingly vast number of different antibody molecules are needed. The amino acids making up protein molecules can certainly …

Antibodies, Antigens, and Epitopes

Antigen The term antigen refers to any foreign molecule that provokes a response by the immune system. In practice, most antigens are proteins made by invading bacteria or viruses. In particular, glycoproteins, which carry carbohydrate residues, and lipoproteins, which carry lipid …

Antibody Structure and Function

The world is full of infectious microorganisms, all looking for a suitable host to infect. Bacteria, viruses, and protozoans are constantly attempting to gain entry into our tissues. If nothing was done about these attempts at invasion, no human could …

BOTOX—BOTULINUM TOXIN

Botulinum toxin is the most toxic substance known. It is made by the anaerobic bacterium, Clostridium botulinum , and is the cause of botulism, a severe form of food poisoning. It has been proposed as a biowarfare agent but has …

DNA Fingerprinting

DNA fingerprinting relies on the unique pattern made by a series of DNA fragments after separating them according to length by gel electrophoresis. DNA samples from different suspects, the victim, and samples from the crime scene are first purified. Restriction …